C7354
Sigma
Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| MDL number: | MFCD00164553 |
Description
| Analysis Note | Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining. |
| Immunogen | synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 28 kDa |
| species reactivity | chicken, rat, human |
| application(s) | immunoblotting: 1:1,000 using recombinant rat calbindin-D-28K |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections | |
| microarray: suitable | |
| Gene Information | human ... CALB1(793) |
| rat ... Calb1(83839) | |
| mouse ... Calb1(12307) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Hassinger, L., et al., Subpopulations of Neurons Expressing Parvalbumin in the Human Amygdala J. Comp. Neurol. 496, 706-722, (2006) Abstract |
